【英文标准名称】:StandardTestMethodsforSulfate-ReducingBacteriainWaterandWater-FormedDeposits
【原文标准名称】:水和水形成沉积物中硫酸盐还原菌的标准试验方法
【标准号】:ASTMD4412-1984(2002)
【标准状态】:现行
【国别】:
【发布日期】:1984
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:硫酸盐还原细菌;沉积物;细菌纲;试验;水
【英文主题词】:water;water-formeddeposits;sulfate;sulfate-reducingbateria
【摘要】:Sulfate-reducingbacteriaarewidelydistributedinmarineandfreshwatermudswhich,inconsequence,frequentlyareladenwiththehydrogensulfideproducedbytheseorganismsduringdissimilatorysulfatereduction.IthasbeenreportedthatDesulfovibriocanformasmuchas10gofsulfideperlitreduringactivemultiplication.Sulfate-reducingbacteriacancausetheexternalorinternalcorrosionofwaterorwastewaterpipelinesandpipelinesforpetroleumandnaturalgas.Theformationofgalvaniccellsbymassivegrowthofsulfate-reducingbacteriaundersuitableconditionsmakesthecorrosionmuchworsethanjusttheeffectofthehydrogensulfideonthemetalorconcrete.1.1Thesetestmethodscovertheprocedureforthedetectionandenumerationbythemostprobablenumber(MPN)techniqueofsulfate-reducingbacteriainwaterorwater-formeddeposits.1.2Twomediapreparationsareprovided.MediumAwhichispreparedwithreagentgradewater,andMediumBwhichispreparedusingthewatertobesampledasthewatersource.MediumBisofferedforthosespecialconditionswheresulfate-reducingbacterialstrainshaveadaptedtoatypicalnon-freshwaterenvironment.1.3Fortheisolationandenumerationofthermophilicsulfate-reducingbacteriaencounteredinwatersassociatedwithoilandgasproduction,allbroths,dilutionblanks,andincubationsmustbemaintainedattemperaturesofatleast45176;Candpreferablywithin5176;Catthesampletemperature.1.4Thesensitivityofthesetestmethodscanbeincreasedbypurgingthedilutionblanksandtubesofmediawithnitrogenimmediatelypriortouse.1.5TheanalystshouldbeawarethatadequatecollaborativedataforprecisionandbiasstatementsasrequiredbyPracticeD2777arenotprovided.SeeSection11fordetails.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:07_100_20(MICROBIOLOGYOFWATER)
【页数】:3P.;A4
【正文语种】: